Canine Distemper Virus Ag Test Kit (CDV-Ag)

This kit is used to detect canine distemper virus antigen in ocular secretions, nasal fluid and saliva.

Test Principle

Rapid immunochromatographic detection technology for the detection of canine distemper virus antigens. After adding the samples of eye secretions, nasal fluid and saliva to the sample wells, they move along the chromatographic membrane together with the colloidal gold-labeled anti-CDV monoclonal antibody. If the CDV antigen is present in the sample, it will bind to the antibody on the detection line (T) and display a wine red color. No color reaction occurs if no CDV antigen is present in the sample.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Precision: There are no more than one false negative and false positive judgment results within a batch; the color development of the test line and the control line between the three batch number test strips (cards) between batches should be uniform, and the color development between batches is not uniform. There is no more than one judgment result.

Stability: 2-30 ℃ unopened validity period of 2 years

Components 

Sample Requirements

• This kit is only suitable for the detection of ocular secretions, nasal fluids, and saliva samples.
• Test samples Collect fresh samples and put them in the diluent immediately. The diluted samples should be tested as soon as possible within 1 hour.
• If it cannot be detected in time, the diluted samples can be stored at 2-8°C for 24 hours and at -20°C for 1 month

Detection Method

1. Disposable cotton swabs were used to collect eye secretions, nasal fluids, and saliva samples. After collection, the samples were placed in the diluent and stirred for 1 min. The cotton swabs were taken out, the lids were tightened, and the samples were inverted and mixed.
2. Tear open the aluminum foil bag of the test card, take out the test card, and place it on a flat and clean table.
3. Pipette 3 drops (about 80 μl) of the diluted sample into the sample hole of the test card and wait for the test result.
4. Wait 10-15 minutes at room temperature to interpret the test results, and the test results after more than 20 minutes are invalid.

Remarks: The above interpretation time is carried out at room temperature of 15-30°C. If the temperature is lower than 15°C, the interpretation time should be appropriately extended; if the temperature is higher than 30°C, the interpretation time should be appropriately shortened.

Interpretation of Results

Negative: Within the detection window, only the control line (C) has a purple-red band.

Positive: Within the detection window, both the test line (T) and the control line (C) appear purple-red bands.

Invalid: In the detection window, the control line (C) does not appear purple-red band.

Precautions for using the Canine Distemper Virus Antigen Test Kit

1. Please read the instructions carefully before the test. The various reagents provided by this product are only for this test.
2. Products with expired or damaged aluminum foil bags cannot be used.
3. If the kit is taken out under low temperature storage, it needs to be returned to room temperature before opening, and the opened test card should be used as soon as possible to avoid moisture, and it should not exceed 1 hour.
4. Avoid touching the sample well on the test card and the chromatographic strip at the test window.
5. The waste after the test should be regarded as pollutants and properly disposed of according to relevant local regulations.

Limitations

This kit can only detect whether there is canine distemper virus antigen in the tested sample.

The test results of this kit are for clinical reference only and are not used as a basis for diagnosis.