Feline Calicivirus Ag Test Kit (FCV-Ag)

Feline calicivirus is classified as Caliciviridae and Calicivirus. The virus reproduces in the brain plasma, sometimes in a crystalline or beaded arrangement. The virus is resistant to lipid solvents (ether, chloroform and deoxycholine salts); inactive at pH 3, stable at pH 4-5; inactivated at 50°C for 30 minutes. The main manifestations are upper respiratory tract symptoms, namely depression, serous and mucous rhinorrhea, conjunctivitis, stomatitis, tracheitis, bronchitis, accompanied by bipolar fever. Feline calicivirus infection is a frequently-occurring disease in cats with high morbidity and low mortality. This kit is used to detect feline calicivirus antigens in cat eye, nose and mouth secretions.

Test principle

Rapid immunochromatographic detection technology for the detection of feline calicivirus antigens. After the cat’s eye nose and mouth secretion sample was added to the sample well, it moved along the chromatographic membrane together with the colloidal gold-labeled anti-FCV monoclonal antibody. If the FCV antigen is present in the sample, it will bind to the antibody on the detection line (T) and display a red color. No color reaction occurs if no FCV antigen is present in the sample.

Product performance index

Sensitivity≥95%
Specificity≥97.5%
Precision: There are no more than one false negative and false positive judgment results within a batch; the color development of the test line and the control line between the three batch number test strips (cards) between batches should be uniform, and the color development between batches is not uniform. There is no more than one judgment result.
Stability: 2-30 ℃ unopened validity period of 2 years

Storage conditions and validity period

Store at 2-30°C, valid for 24 months.

Components

Sample requirements

• This kit is only suitable for the detection of cat’s eye nose and mouth secretion samples.
• Test samples Collect fresh samples and put them in the diluent immediately. The diluted samples should be tested as soon as possible within 1 hour.
• If it cannot be detected in time, the diluted samples can be stored at 2-8°C for 24 hours and at -20°C for 1 month.

Detection method

• Disposable cotton swabs were used to collect samples of cat’s eye, nose and mouth secretions. After collection, the samples were placed in the diluent and stirred for 1 min. The cotton swab was taken out, the lid was tightened, and the mixture was inverted and mixed.
• Tear open the aluminum foil bag of the test card, take out the test card, and place it on a flat and clean table.
• Pipette 3 drops (about 80 μl) of the diluted sample into the sample hole of the test card and wait for the test result.
• Wait 10-15 minutes at room temperature to interpret the test results, and the test results after more than 20 minutes are invalid.
• Remarks: The above interpretation time is carried out at room temperature of 15-30°C. If the temperature is lower than 15°C, the interpretation time should be appropriately extended; if the temperature is higher than 30°C, the interpretation time should be appropriately shortened.

Test results

Negative: Only the control line (C) has a purple-red band.
Positive: The test line (T) and the control line (C) appear purple-red bands at the same time.
Invalid: The control line (C) does not show a magenta band.

Precautions

• Please read the instructions carefully before the test. The various reagents provided by this product are only for this test.
• Products with expired or damaged aluminum foil bags cannot be used.
• If the kit is taken out under low temperature storage, it needs to be returned to room temperature before opening, and the opened test card should be used as soon as possible to avoid moisture, not more than 1 hour.
• Avoid touching the sample well on the test card and the chromatographic strip at the test window.
• The waste after the test should be regarded as pollutants and properly disposed of according to relevant local regulations.