Assay: Micro-droplet digital PCR technology
Samples: Rash, scab, blister fluid, pustular fluid, whole blood, throat swab specimen
Application: Individual or laboratory nucleic acid testing for monkeypox virus
Detection time: 50 minutes
Huachenyang Monkeypox Virus PCR Test Kit was registered by the UK MHRA and can be sold in the UK and countries that recognize the UK MHRA registration.
Features
Monkeypox virus nucleic acid detection kit (PCR), with high sensitivity and good specificity, can quickly and accurately identify monkeypox virus by specifically detecting the gene fragments of monkeypox virus, and the test results can be obtained in a short time, which helps the accurate diagnosis and prevention of monkeypox virus.
- The specificity and sensitivity have been significantly improved.
- The absolute quantification index can be obtained directly without relying on internal reference and standard curve.
- The microreaction units are independent and closed to each other, avoiding mutual interference between PCR inhibitors and amplification products of different nucleic acid molecules, with higher accuracy and reproducibility.
Digital PCR Results
A minimum quantification limit of 5 copies/μl can be achieved with this monkeypox virus micro-droplet digital PCR test kit.
A: Quantification result of 5 copies/μl for surface positive samples.
B: Fluorescence photograph of droplet generation of positive samples with good droplet generation.
C: 1D scatter plot of the positive sample. The positive signal is well separated from the negative signal, and there is no rain-drop effect.
Fluorescent Quantitative PCR Results
The monkeypox virus micro-droplet digital PCR test kit is also compatible for use with common fluorescence quantitative PCR instruments, achieving a minimum quantification limit of 50 copies/μl on the ABI7500 fluorescence quantitative PCR.
Cat. No. List
REF No. | CY-MNA-24 | CY-MNA-48 | CY-MNA-96 | CY-MNA-480 | CY-MNA-960 |
---|---|---|---|---|---|
Reaction buffer A | 459 μl/tube (1tube) | 918 μl/tube (1tube) | 918 μl/tube (1tube) | 4590 μl/tube (2tubes) | 4590 μl/tube (4tubes) |
Reaction buffer B | 102 μl/tube (1tube) | 204 μl/tube (1tube) | 204 μl/tube (2tubes) | 1020 μl/tube (2tubes) | 1020 μl/tube (4tubes) |
Positive control | 20 μl/tube (1tube) | 40 μl/tube (1tube) | 80 μl/tube (1tube) | 200 μl/tube (2tubes) | 200 μl/tube (4tubes) |
Negative control | 20 μl/tube (1tube) | 40 μl/tube (1tube) | 80 μl/tube (1tube) | 200 μl/tube (2tubes) | 200 μl/tube (4tubes) |